Oligoasthenoteratozoospermic (OAT) men display altered phospholipase C ζ (PLCζ) localization and a lower percentage of sperm cells expressing PLCζ and post-acrosomal sheath WW domain-binding protein (PAWP)
نویسندگان
چکیده
منابع مشابه
Relationship between Potential Sperm Factors Involved in Oocyte Activation and Sperm DNA Fragmentation with Intra-Cytoplasmic Sperm Injection Clinical Outcomes
OBJECTIVE The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS In this experimental study,...
متن کاملFunctional disparity between human PAWP and PLCζ in the generation of Ca2+ oscillations for oocyte activation.
Mammalian oocyte activation is mediated by cytosolic calcium (Ca(2+)) oscillations initiated upon delivery of a putative 'sperm factor' by the fertilizing sperm. Previous studies suggest the identity of this sperm factor as the testis-specific phospholipase C-zeta (PLCζ). Recently, a post-acrosomal sheath WW domain-binding protein (PAWP) has been proposed as an alternative sperm factor candidat...
متن کاملI-15: Assessment of Transcript and Protein Profiles of Infertile Individual May Help to Select Individuals with Low Fertilization Potential Candidate of Artificial Oocyte Activation
Background Following sperm penetration, oocyte is activated by sperm oocyte activating factors (SOAFs) released by sperm. Spermspecific phospholipase C isoform ζ (PLCζ) and post acrosomal WW binding protein (PAWP) are two nominees for the SOAF. PLCζ is located back-to-back with another testis-specific gene called CAPZA3. These two genes share a common bidirectional promoter. In this study we as...
متن کاملSperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.
Mature mammalian oocytes undergo a prolonged series of cytoplasmic calcium (Ca(2+)) oscillations at fertilization that are the cause of oocyte activation. The Ca(2+) oscillations in mammalian oocytes are driven via inositol 1,4,5-trisphosphate (IP3) generation. Microinjection of the sperm-derived phospholipase C-zeta (PLCζ), which generates IP3, causes the same pattern of Ca(2+) oscillations as...
متن کاملrelationship between potential sperm factors involved in oocyte activation and sperm dna fragmentation with intra-cytoplasmic sperm injection clinical outcomes
objective: the present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath ww domain-binding protein (pawp), phospholipase cζ (plcζ), and truncated form of the kit receptor (tr-kit)] as candidates of oocyte activation with fertilization rate and early embryonic development. materials and methods: in this experimental study,...
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ژورنال
عنوان ژورنال: Bosnian Journal of Basic Medical Sciences
سال: 2017
ISSN: 1840-4812,1512-8601
DOI: 10.17305/bjbms.2017.2208